Optimization of Surface Sterilization and Organogenesis Protocol for Monstera spp.

Angelina Putri Indi Tjahjono, Listya Utami Karmawan

Abstract


In vitro micropropagation provides faster and more economical solutions to increase world ornamental plant demands. The present study focuses on surface sterilization and multiple shoot induction of Monstera adansonii and M. deliciosa. Combination of sterilant agent such as 70% ethanol (1 min) + 10% NaClO (5 min) + 5% NaClO (5 min) + 2% NaClO (5 min) has highest M. deliciosa (80.53%) and M. adansonii (65.77%) culture viability. Meanwhile, the highest callus formation on M. deliciosa was achieved in Murashige and Skoog medium supplemented with 0.5 ppm BAP + 0.1 ppm TDZ. Taken all together, this study achieved a sterilization protocol and callus induction of M. deliciosa and could be considered for large-scale multiplication. In addition, serve as a base for further detailed analysis of organogenesis induction for both Monstera species


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References


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